il 27 p28 antibody  (Bio X Cell)

Journal: Nature Communications

Article Title: Interleukin-27 is antiviral against Zika virus at the maternal-fetal interface

doi: 10.1038/s41467-025-67378-0

Figure Lengend Snippet: A Timeline for mating and treatment of human STAT2 knock-in (hSTAT2 KI) mice with high molecular weight polyinosinic-polycytidylic acid (HMW poly(I:C)). Created in BioRender. Jurado, K. (2025) https://BioRender.com/eegilr9 . B Serum IL-6 levels in PBS- and HMW Poly(I:C)-treated dams at 6 h post-injection as determined via ELISA. n = 3 dams per group. Statistical analysis performed via ordinary two-way ANOVA, **p < 0.01, ns= not significant (From left, p = >0.9999, 0.0013, 0.0011, 0.9896). Data presented as mean values ± SD. C Proportion of fetuses exhibiting healthy, early resorption, or total resorption phenotypes at E13.5 in PBS- and HMW Poly(I:C)-treated dams. Graph displays total number of fetuses from 3-4 litters per condition. D Representative images depicting fetal outcomes at E13.5. Phenotypes were determined for each fetus based on gross morphology and tissue integrity, as described in methods. E Timeline for mating and infection of hSTAT2 KI mice, as described in methods. Created in BioRender. Jurado, K. (2025) https://BioRender.com/m1xcoc2 . F Proportion of fetuses exhibiting healthy, early resorption, or total resorption phenotypes at E13.5 in PBS- and ZIKV-infected dams. Fetal outcomes were evaluated in 3 independent litters for all conditions except ZIKV-infected IL27RA −/− dams, for which 4 independent litters were evaluated. Graph displays total number of fetuses per condition. G–I ZIKV burdens at E13.5 as determined via quantitative RT-PCR. G Combined ZIKV burden of one matching fetus and placenta, normalized to combined tissue weight. Shape of data point represents observed fetal phenotype. Total number of fetal/placental units per condition plotted (see 4 F ). Statistical analysis performed via Kruskal-Wallis ANOVA, ***p < 0.001, ns=not significant (From left, p = >0.9999, 0.0006). H Left: Fetal ZIKV burdens, normalized to fetus weights. Viral burdens for subset of fetuses exhibiting “healthy” phenotypes plotted (Isotype n = 24, α-IL-27 n = 16, IL27RA −/− n = 28). Right: Placental ZIKV burdens, normalized to placental weights. Viral burdens of placentas matched to fetuses with “healthy” phenotypes plotted (Isotype n = 24, α-IL-27 n = 16, IL27RA −/− n = 28). Statistical analysis performed via Kruskal-Wallis ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns=not significant (Fetal from left, p = >0.9999, 0.0374; Placental from left, p = 0.0378, <0.0001). I Maternal serum ZIKV burdens, normalized to serum weight. n = 3 dams for Isotype and α-IL-27-treated groups, n = 4 IL27RA −/− dams. Not significant via Kruskal-Wallis ANOVA.

Article Snippet: Beginning at embryonic day 3.5 (E3.5), pregnant hSTAT2 KI dams were administered 500μg of α-IL-27 (BioXCell, BE0326, clone MM27.7B1) or isotype control (BioXCell, BE0085, clone C1.18.4) antibody via intraperitoneal injection, with subsequent treatments every three days (E6.5, E9.5, and E12.5).

Techniques: Knock-In, High Molecular Weight, Injection, Enzyme-linked Immunosorbent Assay, Infection, Quantitative RT-PCR

Journal: bioRxiv

Article Title: Oncolytic HSV-IL27 expression improves CD8 T cell function and therapeutic activity in syngeneic glioma models

doi: 10.1101/2025.05.12.653429

Figure Lengend Snippet: (A) Schematic illustration of C134 and C027. C134 is a second-generation oHSV with deletion of the γ134.5 genes and insertion of the human cytomegalovirus (HCMV) IRS1 gene in the UL3/UL4 intergenic region. C027 was generated from C134 by insertion of bicistronic copies of murine IL-27 (mIL27) in the deleted γ134.5 regions under the MND promoter. (B) mIL27 (p28) secretion from C027 or C134 infected (MOI=1) CT-2A cell supernatants at 24- and 48-hours post-infection measured by ELISA. (C) Viral replication in CT-2A cells infected with C027, C134, or WT HSV (MOI=1) demonstrating viral replication kinetics. Experimental design schematics and representative Kaplan-Meier survival curves in (D) CT-2A (n=11 per cohort), (F) KR158 (n=5- 10 per cohort), and (H) SB28 (n=10 per cohort) immunocompetent syngeneic murine glioma models (Log- Rank test of significance) after treatment with Saline or equivalent PFU (1×10 7 ) of C027 or C134 (parental control). In vivo viral recovery from murine brain tumors (E) CT-2A, (G) KR158, and (I) SB28 at two days post- treatment with C134 (n=3) or C027 (n=3). For B, C, E, G, and I, data are mean ± SEM with each shape representing one replicate or animal. Statistical analyses were performed using two-way analysis of variance with Holm-Šídák’s correction for multiple comparisons (B), unpaired two-tailed Student’s t-test (E, G, and I), or Log-Rank tests of significance (D, F, and H). IC, intracranial; oHSV, oncolytic herpes simplex virus; PFU, plaque forming unit; MOI, multiplicity of infection; IL, interleukin; Tx, treatment.

Article Snippet: Antibodies used for in vivo experiments include: anti-CD8a (2.43, Leinco), anti-IL27 p28 (MM27.7B1, BioXCell), rat IgG2b isotype (LTF-2, BioXCell), mouse IgG2a isotype (N123, Leinco) and were administered by intraperitoneal injection on indicated days.

Techniques: Generated, Infection, Enzyme-linked Immunosorbent Assay, Saline, Control, In Vivo, Two Tailed Test, Virus

Journal: iScience

Article Title: Interferon-γ and IL-27 positively regulate type 1 regulatory T cell development during adaptive tolerance

doi: 10.1016/j.isci.2025.112308

Figure Lengend Snippet:

Article Snippet: Mouse Anti-Mouse IL-27 p28 (clone MM27.7B1) , BioXCell , Cat #BE0326; RRID:AB_2819053.

Techniques: Recombinant, Saline, Staining, Red Blood Cell Lysis, Sequencing, Software